Methods and Techniques

1. Adaptive Laboratory Evolution (ALE):
   • Evolutionary strategies to adapt cells for non-standard biochemical functions.
   • Integration of ALE with genome editing and metabolic engineering.
2. Directed Evolution:
   • Classical and machine learning-assisted approaches for enzyme and cell evolution.
   • Libraries for gene editing targeting synthetic metabolism and biocatalysis.
3. Orthogonal Enzymes and Translation Systems:
   • Creation of specialized enzymes for bioorthogonal chemistry and biocatalysis.
   • Novel translation machinery supporting non-standard biochemical processes.
4. Genome Editing & Gene Integration:
   • Advanced techniques for precise genetic modifications.
   • Functional integration of synthetic genes into natural systems.

The research in Laboratory for Chemical Synthetic Biology and Xenobiology is anchored in a highly interdisciplinary approach, merging chemistry, biology, physics, and materials science. By combining these methods, our team engineer innovative technologies, such as synthetic cells and protein-based materials, while investigating fundamental questions about life. This cross-disciplinary work drives impactful solutions in biotechnology and beyond.

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Cross-Disciplinary Integration

1. Biophysics and Biophysical Chemistry:
   • Detailed studies of protein structure, function, and dynamics under varying conditions.
   • Exploration of novel biochemical interactions enabled by ncAAs.
2. Classical Organic and Peptide Synthesis:
   • Leveraging traditional synthetic chemistry techniques to integrate ncAAs into biological systems.
3. Social, Cultural, and Ethical Dimensions:
   • Addressing societal implications of xenobiology and synthetic biology.
   • Promoting awareness and education regarding the transformative potential of these fields.

Further Reading:

Explore Science in Action with Our Lab!

Watch our visually engaging, easy-to-follow videos showcasing groundbreaking science, some methods and innovative projects, featured in the JoVE (Journal of Visualized Experiments).

• Engineering 'Golden' Fluorescence: Non-Canonical Amino Acids & Protein Analysis l Protocol Preview
• Antimicrobial Peptides Produced: Non-Canonical Amino Acids l Protocol Preview