Publications

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An Engineered Escherichia coli Strain with Synthetic Metabolism for In-cell Production of Translationally Active Methionine Derivatives

ChemBioChem 2020 , 21(24), 3525-3538

Schipp, C. J., Ma, Y., Al-Shameri, A. Contestabile, R, Budisa*, N. and DiSalvo*, M.L.

Expanding the Scope of Orthogonal Translation with Pyrrolysyl-tRNA Synthetases Dedicated to Aromatic Amino Acids.

Molecules 2020 , 25(19), 4418

Tseng, H.W., Baumann, T., Sun, H., Wang, Y. S., Ignatova, Z. and Budisa, N.

Courses Based on iGEM/BIOMOD Competitions Are the Ideal Format for Research‐Based Learning of Xenobiology.

ChemBioChem 2020 , Online ahead of print

Schmitt, F.-J., Frielingsdorf, S. Friedrich, T. and Budisa, N.

Multi-omics analysis provides insight into the laboratory evolution of Escherichia coli towards the metabolic usage of fluorinated indoles.

ACS Cent. Sci 2020 , Online ahead of print

Agostini, F., Sinn, L., Petras, D., Schipp, C.J., Kubyshkin, V., Berger, A.A., Rappsilber, J., Dorrestein, P.C., Budisa, N. and Koksch, B.

Conjugation of Synthetic Polyproline Moietes to Lipid II Binding Fragments of Nisin Yields Active and Stable Antimicrobials.

Front. Microbiol. 2020 , 11:575334

Deng, J., Viel, J-H., Kubyshkin, V., Budisa, N. and Kuipers, O.

Combating antimicrobial resistance with new-to-nature lanthipeptides created by genetic code expansion.

Front. Microbiol. 2020 , 1:590522

Heidari-Karbalaei, H. R. and Budisa, N.

Fine‐tuning protein self‐organization by orthogonal chemo‐optogenetic tools

Angew Chem Int Ed Engl 2020 , Online ahead of print

Sun, H., Jia, H., Ramirez-Diaz, D. A., Budisa, N. and Schwille, P.

Discovery and characterization of a new cold-active protease from an extremophilic bacterium via comparative genome analysis and in vitro expression

Front. Microbiol. 2020 , 11:881

Perfumo, A., von Sass, G.J.F., Nordmann, E.L., Budisa, N. and Wagner, D.

Xenobiology: a journey towards parallel life forms

ChemBioChem 2020 , 21(16), 2228-2231

Budisa, N., Kubyshkin, V. and Schmidt, M.

Phage capsid nanoparticles with defined ligand arrangement block influenza virus entry - an antiviral strategy

Nat Nanotechnol 2020 , 15, 373-379

Lauster, D., Klenk, S., Ludwig, K.; &, e. al, Budisa, N., Netz., R., Böttcher, C., Liese, S., Herrmann, A. and Hackenberger, C.P.

Synthesis of New Aza- and Thia-Crown Ether Based Amino Acids

ChemistrySelect 2020 ,5(9), 2854-2857

Schneider, T., Brüssow, N., Yuvanc, A., Budisa, N.

Student-centred research-based projects are highly motivating

84th DPG Spring Meeting of the German Physical Society 2020

Schmitt, F.-J., Budisa, N. and Krause-Rehberg, R.

Alternative Biofacts - Life as we don't (yet) know it

Art as we don’t know it. 2020 , Book published by Aalto University Publication Series: Helsinki; pp 75-83

Budisa, N., Schmidt, M.

The Alanine World Model for the Development of the Amino Acid Repertoire in Protein Biosynthesis

Int. J. Mol. Sci. 2019 ,20(21), e5507

Kubyshkin, V., Budisa, N.

Bilayer thickness determines the alignment of model polyproline helices in lipid membranes

Phys Chem Chem Phys. 2019 ,21(40), 22396-22408

Kubyshkin, V., Grage, S.L., Ulrich, A.S., Budisa N.

Alternative Biochemistries for Alien Life: Basic Concepts and Requirements for the Design of a Robust Biocontainment System in Genetic Isolation

Genes 2019 ,10(1),17

Diwo. C, Budisa, N.

Site-Specific Chemoselective Pyrrolysine Analogues Incorporation Using the Cell-Free Protein Synthesis System

ACS Synth. Biol. 2019 ,8(2), 381-390

Gerrits, M., Merk, H., Budisa, N.

In-Cell Synthesis of Bioorthogonal Alkene Tag S-Allyl-Homocysteine and Its Coupling with Reprogrammed Translation

Int. J. Mol. Sci. 2019 ,20(2), 2299

Nojoumi, S., Ying, M., Schwagerus, S., Hackenberger, C.P.,R., Budisa, N.

Site-Resolved Observation of Vibrational Energy Transfer Using a Genetically Encoded Ultrafast Heater

Angew. Chem. Int. Ed. Engl. 2019 ,58(9), 2899-2903

Baumann, T., Hauf, M., Schildhauer, F., Eberl, K.B., Durkin, P.M., Deniz, E., Löffler, J.G., Acevedo-Rocha, C.G., Jaric, J., Martins, B.M., Dobbek, H., Bredenbeck, J., Budisa, N.

Computational Aminoacyl-tRNA Synthetase Library Design for Photocaged Tyrosine

Int. J. Mol. Sci. 2019 ,20(9), 2343

Baumann, T., Hauf, M.; Richter, F., Albers, S., Möglich, A., Ignatova, Z., Budisa, N.

Promotion of the collagen triple helix in a hydrophobic environment

Org. Biomol. Chem. 2019 ,17(2), 2502-2507

Kubyshkin, V., Budisa, N.

Anticipating alien cells with alternative genetic codes: away from the alanine world!

Curr. Opin. Biotechnol. 2019 ,60, 242-249

Kubyshkin, V., Budisa, N.

Expanding the DOPA Universe with Genetically Encoded, Mussel-Inspired Bioadhesives for Material Sciences and Medicine

ChemBioChem 2019 2019 ,20, 1-29

Schneider, T., Budisa, N.

Synthesis of a new metal chelating amino acid: Terpyridyl-alanine

Tetrahedron Lett. 2019 ,2018(19), 2053-2063

Schneider, T.; Budisa, N.

Accounting for a High Technology - Fourth Genetic Technology Report of Interdisciplinary Research Group of the Berlin-Brandenburg Academy of Sciences and Humanities

Nomos Verlag, Germany 2018 ,203-236

Budisa, N., B Fehse, B.; Müller-Röber, B., Reich, J.; Walter, J.

Self-Directed in Cell Production of Methionine Analogue Azidohomoalanine by Synthetic Metabolism and Its Incorporation into Model Proteins

Mol. Biol. 2018 (1),127-135

Ma, Y., Salvo, M. L., Budisa, N.

Painting argyrins blue: Negishi cross-coupling for synthesis of deep-blue tryptophan analogue ß-(1-azulenyl)-l alanine and its incorporation into argyrin C.

Bioorg. Med. Chem. 2018 , in Press.

Stempel E., Kaml R. F., Budisa, N., Kalesse, M.

Long-Range Modulations of Electric Fields in Proteins

J. Phys. Chem. B 2018 B122(35),8330-8342

Biava, H.; Schreiber, T., Katz, S., Vo¨ller, J-S., Stolarski, M., Schulz, C., Michael, N., Budisa, N., Kozuch, J., Tillmann, U., Hildebrandt, P.

Synthesis of a Photo-Caged DOPA Derivative by Selective Alkylation of 3,4-Dihydroxybenzaldehyde

Eur. J. Org. Chem. 2018 2018(18),2053-2063

Schneider, T., Kubyshkin, V., Budisa, N.

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids.

J. Vis. Exp. 2018 e57551(135)

Nickling, J. H., Baumann, T., Schmitt, F. J., Bartholomae, M., Kuipers, O. P., Friedrich, T., Budisa, N.

Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence.

J. Vis. Exp. 2018 e57017(134)

Baumann, T., Schmitt, F. J., Pelzer, A., Spiering, V. J., Freiherr von Sass, G. J., Friedrich, T., Budisa, N.

Transmembrane Polyproline Helix

Phys. Chem. Lett. 2018 9(9),2170-2174

Kubyshkin, V., Grage, S. L., Bürck, J., Ulrich, A. S., Budisa, N.

Expanding the genetic code of Lactococcus lactis and Escherichia coli to incorporate non-canonical amino acids for production of modified lantibiotics

Front. Microbiol. 2018 9,657

Bartholomae, M., Baumann, T., Nickling, J.H., Peterhoff, D., Wagner, R., Budisa, N., Kuipers, O. P.

Exploring hydrophobicity limits of polyproline helix with oligomeric octahydroindole-2-carboxylic acid

J. Pept. Sci. 2018 24(4-5),e3076

Kubyshkin, V., Budisa, N.

“Comparative effects of trifluoromethyl-and methyl-group substitutions in proline

New J. Chem. 2018 , 42, 13461-13470

Kubyshkin, V., Pridma, S., Budisa, N.

Comprehensive identification of proteins binding to RNA G-quadruplex motifs in the 5' UTR of tumor-associated mRNAs

Biochimie 2018 , 144, 169-184

Serikawa, T., Spanos, C., vonHacht, A., Budisa, N., Rappsilber, J., Kurreck, J.“Comparative effects of trifluoromethyl-and methyl-group substitutions in proline

Discovery and Investigation of Natural Editing Function against Artificial Amino Acids in Protein Translation

ACS Cent. Sci. 2017 ,3 (1),73-80

Jan-Stefan Völler, Morana Dulic, Ulla I. M. Gerling-Driessen, Hernan Biava, Tobias Baumann, Nediljko Budisa*, Ita Gruic-Sovulj*, and Beate Koksch*

Biocatalysis with Unnatural Amino Acids: Enzymology Meets Xenobiology

Angew. Chem. Int. Ed. 2017, 56, 2-26

Federica Agostini, Jan-Stefan Völler, Beate Koksch, Carlos G. Acevedo-Rocha, Vladimir Kubyshkin and Nediljko Budisa

Amide rotation trajectories probed by symmetry

Biomol. Chem. 2017, 15, 6764-6772

Vladimir Kubyshkin and Nediljko Budisa

Deciphering the Fluorine Code—The Many Hats Fluorine Wears in a Protein Environmenty

Acc. Chem. Res. 2017, 50(9), 2093-2103

Allison Ann Berger, Jan-Stefan Völler, Nediljko Budisa and Beate Koksch

Synthetic alienation of microbial organisms by using genetic code engineering: Why and how?

Biotechnol. J. 2017, 12(8),

Vladimir Kubyshkin and Nediljko Budisa

Construction of a polyproline structure with hydrophobic exterior using octahydroindole-2-carboxylic acid

Org. Biomol. Chem. 2017, 15, 619-627

Vladimir Kubyshkin and Nediljko Budisa

Künstliche Evolution des genetischen Codes von Mikroorganismen (In German)

BIOspektrum 2017, 21, 146-149

Jan-Stefan Völler, M. G. Hoesl and Prof. Dr. Nediljko Budisa

An expanded genetic code for probing the role of electrostatics in enzyme catalysis by vibrational Stark spectroscopy

Biochim. Biophys. Acta 2017, 1861, 3053-3059

Jan-Stefan Völler, Hernan Biava, Peter Hildebrandt and Nediljko Budisa

Coupling genetic code expansion and metabolic engineering for synthetic cells

Curr. Op. Biotechnol. 2017, 48, 1-7

Jan-Stefan Völler and Nediljko Budisa

Xenobiology: State-of-the-Art, Ethics, and Philosophy of New-to-Nature Organisms

Synthetic Biology-Metabolic Engineering 301-315

Markus Schmidt, Lei Pei and Nediljko Budisa

Selective 19F-Labeling of Functionalized Carboxylic Acids with Difluoromethyl Diazomethane (CF2HCHN2)

Chem. Eur. J. 2017, 23, 13279-13283

Mykhailiuk, P., Kishko, I., Kubyshkin, V., Budisa, N. and Cossy, J.

On universal coding events in protein biogenesis

Biosystems 2017

Kubyshkin, V., Acevedo-Rocha, C. G. and Budisa, N.

Prospects of In vivo Incorporation of Non-canonical Amino Acids for the Chemical Diversification of Antimicrobial Peptides

Front. Microbiol. 2017, 8, 124

Baumann, T., Nickling, J. H., Bartholomae, M., Buivydas, A., Kuipers, O. P. and Budisa, N.

Broad substrate tolerance of tubulin tyrosine ligase enables one-step site-specific enzymatic protein labeling

Chem. Sci. 2017, 8, 3471-3478

Schumacher, D., Lemke, O., Helma, J., Gerszonowicz, L., Waller, V., Stoschek, T., Durkin, P. M., Budisa, N., Leonhardt, H., Keller, B. and Hackenberger, C.

The Regioselective Synthesis of o-Nitrobenzyl DOPA Derivatives

Synthesis 2017, 49, 2691-2699

Schneider, T., Martin, J., Durkin, P. M., Kubyshkin, V. and Budisa, N.

Peptidyl-Prolyl Model Study: How Does the Electronic Effect Influence the Amide Bond Conformation?

J. Org. Chem. 2017, 82(17), 8831-8841

Mykhailiuk, P., Kubyshkin, V., Bach, T. and Budisa, N.

Hydrolysis, polarity, and conformational impact of C-terminal partially fluorinated ethyl esters in peptide models

Beilstein J. Org. Chem. 2017, 2442-2457

Kubyshkin, V. and Budisa, N.

Comprehensive identification of proteins binding to RNA G-quadruplex motifs in the 5' UTR of tumor-associated mRNAs

Biochemie 2017, 144, 2442-2457

Serikawa T., Spanos C., von Hacht A., Budisa N., Rappsilber J. Kurreck J.

Global substitution of hemeproteins with noncanonical amino acids in Escherichia coli with intact cofactor maturation machinery

Enzyme Microb. Technol. 2017, 106, 55-59

Völler, J.-S., To, T., Biava, H., Koksch, B. and Budisa, N.

Photoactivatable Mussel-Based Underwater Adhesive Proteins by an Expanded Genetic Code

ChembioChem 2017, 18, 1819-1823

Hauf, M., Richter, F., Schneider, T., Faidt, T., Martins, B. M., Baumann, T., Durkin, P., Dobbek, H., Jacobs, K., Moeglich, A. and Budisa, N.

Design of S-Allylcysteine in Situ Production and Incorporation Based on a Novel Pyrrolysyl-tRNA Synthetase Variant

ChembioChem 2017, 85-90

Exner, M. P., Kuenzl, T., Schwagerus, S., To, T., Ouyang, Z., Hoesl, M. G., Lensen, M. C., Hackenberger, C. P. R., Panke, S. and Budisa, N.

Residue-specific Incorporation of Noncanonical Amino Acids into Model Proteins Using an Escherichia coli Cell-free Transcription-translation System

J. Vis. Exp. 2016, e54273

Worst, E. G., Exner, M. P., De Simone, A., Schenkelberger, M., Noireaux, V., Budisa, N. and et al.

One-Pot Synthesis of Unprotected Anomeric Glycosyl Thiols in Water for Glycan Ligation Reactions with Highly Functionalized Sugars

Angew. Chem. Int. Ed. 2016, 55 15510-15514

Köhling, S., Exner, M. P., Nojoumi, S., Schiller, J., Budisa, N. and Rademann, J.

Congeneric lipases with improved catalytic activity and substrate accessibility / Engineering lipases with an expanded genetic code

Applied Biocatalysis From Fundamental Science to Industrial Applications 2016, Wiley-VCH

De Simone, A., Hoesl, M. G. and Budisa, N.

Discharging tRNAs: a tug of war between translation and detoxification in Escherichia coli

Nucleic Acids Res. 2016, 44, 8324-8334

Avcilar-Kucukgoze, I., Bartholomäus, A., Cordero Varela, J., Kaml, R., Neubauer, P., Budisa, N. and Ignatova, Z.

Xenomicrobiology: a roadmap for genetic code engineering

Microb. Biotechnol. 2016, 9, 666-676

Acevedo-Rocha, C. and Budisa, N.

Incorporation of Amino Acids with Long-Chain Terminal Olefins into Proteins

Molecules 2016, 21, 287

Exner, M. P., Köhling, S., Rivollier, J., Gosling, S., Srivastava, P., Palyancheva, Z. I., Herdewijn, P., Heck, M.-P., Rademann, J. and Budisa, N.

cis–trans-Amide isomerism of the 3,4-dehydroproline residue, the ‘unpuckered’ proline

Beilstein J. Org. Chem. 2016, 12, 589-593

Kubyshkin, V. and Budisa, N.

Energetic contribution to both acidity and conformational stability in peptide models

New J. Chem. 2016, 40, 5209-5220

Kubyshkin, V., Durkin, P. M. and Budisa, N.

The New Worlds of Synthetic Biology

Synthetic Biology Analyzed 2016, 44, 1-25

Engelhard, M., Boelker, M., Budisa, N. and Winter, G.

Diverese Layers of Live

Synthetic Biology Analyzed 2016, 44, 27-50

Boelker, M., Engelhard, M. and Budisa, N.

Photochemical formation of quinone methides from peptides containing modified tyrosine

Organic & Biomolecular Chemistry 2016, 14, 10894-10905

Husak, A., Noichl, B., Sumanovac-Ramljak, T., Sohora, M., Skalamera, D., Budisa, N. and Basaric, N.

Design and Application of Autofluorescent Proteins by Biological Incorporation of Intrinsically Fluorescent Noncanonical Amino Acids

Fluorescent Analogs of Biomolecular Building Blocks 2016, Wiley, 91-124

Durkin, P. M. and Budisa, N.

Orthogonal Protein Translation Using Pyrrolysyl-tRNA Synthetases for Single - and Multiple-Noncanonical Amino Acid Mutagenesis

Adv. Biochem. Eng. Biotechnol. 2016, Springer, 1-19

Baumann, T., Exner, M. P. and Budisa, N.

Towards reassignment of the AUG Methionine codon to two different non-canonical amino acids in bacterial translation

Croatica Chemica Acta 2016, 89, 1-19

Baumann, T., Exner, M. P. and Budisa, N.

Towards Biocontained Cell Factories: An Evolutionarily Adapted Escherichia coli Strain Produces a New-to-nature Bioactive Lantibiotic Containing Thienopyrrole-Alanine

Scientific Reports 2016, 6

Kuthning, A., Durkin, P., Oehm, S., Hoesl, M., Budisa, N. and Süssmuth, R.

Covalent Attachment of Cyclic TAT Peptides to GFP Results in Protein Delivery into Live Cells with Immediate Bioavailability

Angew. Chem. Int. Ed. Eng. 2015, 54, 1950-1953

Nischan, N., Herce, H., Natale, F., Bohlke, N., Budisa, N., Cardoso, M. C. and Hackenberger, C. P. R.

Applying ?-substituted prolines in the foldon peptide: polarity contradicts preorganization

ChembioChem 2015, 16, 403-406

Dietz, D., Kubyshkin, V. and Budisa, N.

?-(S)-Trifluoromethyl proline: Evaluation as a structural substitute of proline for solid state 19F-NMR peptide studies

Org. Biomol. Chem. 2015, 13, 3171-3181

Kubyshkin, V., Afonin, S., Kara, S., Budisa, N., Mykhailiuk, P. K. and Ulrich, A. S.

Synthetic Biology: Is a new kind of Biological Engineering emerging?

Third gene technology report: Analysis of a high-tech sector. 2015

Budisa, N. and Hümpel, A.

Orthogonal Translation Meets Electron Transfer: In Vivo Labeling of Cytochrome c for Probing Local Electric Fields

ChembioChem 2015, 16, 742-745

Völler, J., Biava, H., Koksch, B., Hilderbrandt, P. and Budisa, N.

Xenobiologie, künstliches Leben und genetische Firewall

Grenzüberschreitungen - Synthetische Biologie im Dialog. 2015, Verlag Karl Alber

Budisa, N.

Design of orthogonal pairs for protein translation: selection systems for genetically encoding noncanonical amino acids in E. coli

Hydrocarbon and Lipid Microbiology Protocols: Cultivation 2015, Springer-Verlag

Jaric, J. and Budisa, N.

Orthogonal dual-modification of proteins for the engineering of multivalent protein scaffolds

Beilstein J. Org. Chem. 2015, 11, 784-791

Mühlberg, M., Hoesl, M. G., Kuehne, C., Dernedde, J., Budisa, N. and Hackenberger, C. P. R.

Towards Direct Measurement of Ultrafast Vibrational Energy Flow in Proteins

Ultrafast Phenomena XIX 2015

Müller-Werkmeister, H. M., Essig, M., Durkin, P., Budisa, N. and Bredenbeck, J.

Site-specific conjugation of 8-ethynyl-BODIPY to a protein by [2+3] cycloaddition

Org. Biomol. Chem. 2015, 13, 6728-6736

Albrecht, M., Lippach, A., Exner, M. P., Jerbi, J., Springborg, M., Budisa, N. and Wenz, G.

Cell-free expression with the toxic amino acid canavanine

Bioord. Med. Chem. Lett. 2015, 25, 3658-3660

Worst, E. G., Exner, M. P., De Simone, A., Schenkelberger, M., Noireaux, V., Budisa, N. and Ott, A.

Towards intrinsically colored peptides: The synthesis and investigation of the spectral properties of methylated azatryptophans in Trp-cage mutants

Biopolymers Peptide Science 2015, 104, 585-600

Noichl, B. P., Durkin, P. M. and Budisa, N.

Chemical evolution of a bacterial proteome

Angew. Chem. Int. Ed. Engl. 2015, 54, 10030-10034

Hoesl, M. G., Oehm, S., Durkin, P., Darmon, E., Peil, L., Aerni, H.-R., Rappsilber, J., Rinehart, J., Leach, D., Söll, D. and Budisa, N.

Strategy for Enhancement of 13C-Photo-CIDNP NMR Spectra by Exploiting Fractional 13C-Labeling of Tryptophan

J. Phys. Chem. 2015, 119(43), 13934-13943

Eisenreich, W., Joshi, M., Illarionov, B., Kacprzak, S., Lukaschek, M., Kothe, G., Budisa, N., Fischer, M., Bacher, A. and Weber, S.

Dendronylation: Residue-specific chemoselective attachment of oligoglycerol dendrimers on proteins with noncanonical amino acids

Bioorg. Med. Chem. Lett. 2015, 25, 5247-5249

Ma, Y., Thota, B. N. S., Haag, R. and Budisa, N.

Entropic Contribution of Elongation Factor P to Proline Positioning at the Catalytic Center of the Ribosome

J. Am. Chem. Soc. 2015, 137, 12997-13006

Doerfel, L. K., Wohlgemuth, I., Kubyshkin, V., Starosta, A. L., Wilson, D. N., Budisa, N. and V., R. M.

Life at the Speed of Light– From the From the Double Helix to the Dawn of Digital Life. By J. Craig Venter

Angew. Chem. Int. Ed. Engl. 2014, 53, 9421-9422

Budisa, N.

Secretion of Recombinant Archeal lipase Mediated by SVP2 Signal Peptide in Escherichia coli and its optimization by Response Surface Methodology

Protein Expr. Purif. 2014, 101, 84-90

Pournejati, R., Karbalaei-Heidari, H. R. and Budisa, N.

Evolution of fluorinated enzymes: An emerging trend for biocatalyst stabilization

Eng. Life. Sci. 2014, 14, 340-351

Biava, H.,Budisa, N.

Sense codon emancipation for proteome-wide incorporation of noncanonical amino acids: rare isoleucine codon AUA as a target for genetic code expansion

FEMS Microbiol Lett. 2014, 351, 133-144

Bohlke, N. and Budisa, N.

Xenobiology, New-to-Nature Synthetic Cells and Genetic Firewall

Curr. Org. Chem. 2014, 18, 936-943

Budisa, N.

Coupling Bioorthogonal Chemistries with Artificial Metabolism: Intracellular Biosynthesis of Azidohomoalanine and its Incorporation into Recombinant Proteins

Molecules 2014, 19, 1004-1022

Ma, Y., Biava, H., Contestabile, R., Budisa, N. and di Salvo, M. L.

Improved method to retain cytosolic reporter protein fluorescence while staining for nuclear proteins

Cytometry A. 2014, 85, 621-627

Heinen, A., Wanke, F., Moos, S., Attig, S., Luche, H., Pal, P., Budisa, N., Fehling, H., Waisman, A. and Kurschus, F.

Supercritical Carbon Dioxide and Its Potential as a Life-Sustaining Solvent in a Planetary Environment

Life 2014, 4, 331-340

Budisa, N. and Schulze-Makuch, D.

Supercritical Carbon Dioxide and Its Potential as a Life-Sustaining Solvent in a Planetary EnvironmentFluorine-Rich Planetary Environments as Possible Habitats for Life

Life 2014, 4, 374-385

Budisa, N., Kubyshkin, V. and Schulze-Makuch, D.

Site-Directed and Global Incorporation of Orthogonal and Isostructural Noncanonical Amino Acids into the Ribosomal Lasso Peptide Capistruin

ChembioChem 2014, 16, 503-509

Al Toma, R. S., Kuthning, A., Exner, M. P., Denisiuk, A., Ziegler, J., Budisa, N. and Süssmuth, R. D.

Expanded genetic code for the engineering of ribosomally synthetized and post-translationally modified peptide natural products (RiPPs)

Biotechnol. 2013, 4, 591-598

Budisa, N.

Polyoxometalate-stabilized, water dispersible Fe2Pt magnetic nanoparticles

Nanoscale 2013, 6, 2511-2519

Seemann, K., Bauer, A., Kindervater, J., Meyer, M., Besson, C., Luysberg, M., Durkin, P., Pyckhout-Hintzen, W., Budisa, N., Georgii, R., Schneider, C. M. and Kogerler, P.

Directed Manipulation of a Flavoprotein Photocycle

Angew. Chem. Int. Ed. Engl. 2013, 52, 8463-8466

Staudt, H., Hoesl, M. G., Dreuw, A., Serdjukow, S., Oesterhelt, D., Budisa, N., Wachtveitl, J. and Grininger, M.

Non-canonical amino acids as a useful synthetic biological tool for lipase-catalysed reactions in hostile environments

Catal. Sci. Technol. 2013, 3, 1198-1201

Acevedo-Rocha, C., Hoesl, M., Nehring, S., Royter, M., Wolschner, C., Wiltschi, B., Antranikian, G. and Budisa, N.

Biocatalytic synthesis of (2S)-5,5,5-trifluoroleucine and improved resolution into (2S,4S) and (2S,4R) diastereoisomers

Tetrahedron Letters 2013, 54, 3662-3665

Biava, H. and Budisa, N.

Obtention of enantiomerically pure 5,5,5-trifluoro-L-isoleucine and 5,5,5-trifluoro-L-alloisoleucine

J. Fluorine Chem. 2013, 156, 372-377

Biava, H. and Budisa, N.

Was ist Leben?

Wissenschaftliche Verlagsgesellschaft Stuttgart 2012

Budisa, N.

Congeneric Lantibiotics from Ribosomal In Vivo Peptide Synthesis with Noncanonical Amino Acids

Angew. Chem. 2012, 51, 415-418

Oldach, F., Al-Toma, R., Kuthning, A., Caetano, T., Mendo, S., Budisa, N. and Süssmuth, R. D.

Site-selective modification of proteins for the synthesis of structurally defined multivalent scaffolds

Chem. Commun. 2012, 48, 522-524

Artner, L. A., Merkel, L., Bohlke, N., Beceren-Braun, F., Weise, C., Dernedde, J., Budisa, N. and Hackenberger, C. P. R.

Recent advances in genetic code engineering in Escherichia coli

Curr. Opin. Biotechnol. 2012, 23, 751-757

Hoesl, M. G. and Budisa, N.

Performance analysis of orthogonal pairs designed for an expanded eukaryotic genetic code

PloSONE 2012, e31992

Nehring, S., Budisa, N. and Wiltschi, B.

Congeneric bio-adhesive mussel foot proteins designed by modified prolines revealed a chiral bias in unnatural translation

Biotechnology and Bioprocess Engineering 1 2012, 17, 679-686

Larregola, M., Moore, S. and Budisa, N.

Organic fluorine as a polypeptide building element: in vivo expression of fluorinated peptides, proteins and proteomes

Org. Biomol. Chem. 2012, 1, 7241-7261

Merkel, L. and Budisa, N.

Design of protein congeners containing beta-cyclopropylalanine

Mol. BioSyst. 2012, 8, 2719-2723

Acevedo-Rocha, C. G., Geiermann, A. S., Budisa, N. and Merkel, L.

Evaluation of bicinchoninic acid as a ligand for copper(I)-catalyzed azide-alkyne bioconjugations

Org. Biomol. Chem. 2012, 10, 6629-6632

Christen, E., Gubeli, R., Kaufmann, B., Merkel, L., Schoenmakers, R., Budisa, N., Fussenegger, M., Weber, W. and Wiltschi, B.

Synthetische Biologie. Entwicklung einer neuen Ingenieurbiologie?

Berlin-Brandenburgische Akademie der Wissenschaften 2012

Budisa, N.

Conjugation of Proteins by Installing BIO-Orthogonally Reactive Groups at Their N-Termini

PLoSONE 2012, e46741

Soundrarajan, N., Sokalingam, S., Raghunathan, G., Budisa, N., Paik, H. J., Yoo, T. and G., L. S.

PLP-dependent Enzymes: a Powerful Tool for Metabolic Synthesis of. Non-canonical Amino Acids

Beilsten Symposium on Molecular Engineering and Control 2012, Logos Verlag Berlin

Di Salvo, M., Budisa, N. and Contestabile, R.

Synthetic Biology of Autofluorescent Proteins

Fluorescent Proteins I - From Understanding to Design 2011, Springer-Verlag

Hoesl, M., Merkel, L. and Budisa, N.

Code Engineering: Techniken und Anwendungen

Synthetische Biologie – acatech DISKUSSION XII 2011, Springer-Verlag

Budisa, N. and Hoesl, M.

Playing God by artificially synthesizing life in the laboratory – The historical and cultural perception and evaluation by the society

Angew. Chem. Int. Ed. Engl. 2011, 50, 11041-11042

Budisa, N.

Trendbericht Synthetische Biologie: Techniken

Nachrichten aus der Chemie 2011, 59, 309-312

Merkel, L. and Budisa, N.

Genetically Encoded Photocrosslinkers as Molecular Probes To Study G-Protein-Coupled Receptors (GPCRs)

Angew. Chem. Int. Ed. Engl. 2011, 51, 310-312

Beck-Sickinger, A. G. and Budisa, N.

Lipase Congeners Designed by Genetic Code Engineering

ChemcatChem 2011, 3, 213-221

Hoesl, M. G., Acevedo Rocha, C., Nehring, S., Wolschner, C., Royter, M., Wiltschi, B., Budisa, N. and Antranikian, G.

Enhancing the thermal stability of a single-chain Fv fragment by in vivo global fluorination of the proline residues

Mol. Biosys. 2011, 7, 258-265

Edwardraja, S., Sriram, S., Govindan, R., Budisa, N. and Lee, S. G.

Expanding and Engineering the Genetic Code in a Single Expression Experiment

ChemBioChem 2011, 12, 552-555

Hoesl, M. G. and Budisa, N.

In Vivo Incorporation of Multiple Noncanonical Amino Acids into Proteins

Angew. Chem. Int. Ed. Engl. 2011, 50, 2896-2902

Hoesl, M. G. and Budisa, N.

Bioconjugation of L-3,4-Dihydroxyphenylalanine Containing Protein with a Polysaccharide

Bioconjugate Chehm. 2011, 22, 551-555

Ayyadurai, N., Prabhu, N., Deepankumar, K., Jang, Y., Chitrapriya, N., Song, E., Lee, N., Kim, S., Kim, B., Soundrarajan, N., Lee, S., Cha, H., Budisa, N. and Yun, H.

On the Road towards Chemically Modified Organisms Endowed with a Genetic Firewall

Angew. Chem. Int. Ed. Engl. 2011, 50, 6960-6962

Acevedo-Rocha, C. G. and Budisa, N.

Kernaussagen und Handlungsempfehlungen

Gentherapie in Deutschland. Eine interdisziplinäre Bestandsaufnahme 2011, Brandenburgische Akademie der Wissenschaften

Müller-Röber, B., Hucho, F., Budisa, N., Fehse, B., Hampel, J., Köchy, K., Reich, J., Rheinberger, H.-J., Ropers, H.-H., Taupitz, J. and Walter, J.

Synthetic biology of protein folding

ChemPhysChem 2010, 11, 1181-1187

Moroder, L. and Budisa, N.

Nicht-kanonische Aminosäuren in der Synthetischen Biologie

BIOspektrum 2010,3,309-311

Hoesl, M. G. and Budisa, N.

Blue fluorescent amino acids as in vivo building blocks for proteins

ChemBioChem 2010,11,305-314

Merkel, L., Hoesl, M. G., Albrecht, M., Schmidt, A. and Budisa, N.

Residue-specific global fluorination of Candida Antarctica lipase B in Pichia pastoris

Mol. Biosyst. 2010,6,1630-1639

Budisa, N., Wenger, W. and Wiltschi, B.

Parallel Incorporation of Different Fluorinated Amino Acids: On the Way to "Teflon" Proteins

ChemBioChem 2010,11,1505-1507

Merkel, L., Schauer, M., Antranikian, G. and Budisa, N.

In Vivo Double and Triple Labeling of Proteins Using Synthetic Amino Acids

Angew. Chem. Int. Ed. Engl. 2010,49,5446-5450

Lepthien, S., Merkel, L. and Budisa, N.

Azatryptophans as tools to study polarity requirements for folding of green fluorescent protein

J. Peptide Sci. 2010,16,589-595

Hoesl, M. G., Larregola, M., Cui, H. and Budisa, N.

Engineering Protein Sequence Composition for Folding Robustness Renders Efficient Noncanonical Amino acid Incorporations

ChemBioChem 2010, 11,2521-2524

Nagasundarapandian, S., Merkel, L., Budisa, N., Govindan, R., Ayyadurai, N., Sriram, S., Yun, H. and Lee, S.-G.

Fine tuning the N-terminal residue excision by methionine analogs

ChemBioChem 2009,10,217-220

Wiltschi, B., Merkel, L. and Budisa, N.

Den Kode des Lebens erweitern

Spektrum der Wissenschaft 2009,42-45

Budisa, N. and Weitze, M. D.

Protein Iodination by Click Chemistry

ChemBioChem 2009, 10,1149-1151

Dong, S. L., Moroder, L. and Budisa, N.

Design of anti- and pro-aggregation variants to assess the effects of methionine oxidation in human prion protein

Proc. Natl. Acad. Sci. USA, 2009106,7756-7761

Wolschner, C., Giese, A., Kretzschmar, H., Huber, R., Moroder, L. and Budisa, N.

Gold Fluorescent Annexin A5 as a Novel Apoptosis Detection Tool

Cytometry Part A 2009,75,626-633

Kurschus, F., Pal, P. P., Bäumler, P., Jenne, D. E., Wiltschi, B. and Budisa, N.

Engineering green fluorescent proteins using an expanded genetic code

Reviews in Fluoresence 2008, Springer New York,359-386

Pal, P. P. and Budisa, N.

Chemical Protein Engineering: Synthetic and Semisynthetic Peptides and Proteins

Protein Engineering 2008,22,20-64

Merkel, L., Moroder, L. and Budisa, N.

Molecular cloning and sequence analysis of a novel zinc-metalloprotease gene from the Salinivibrio sp. strain AF-2004 and its extracellular expression in E. coli

Gene 2008,408,196-203

Karbalaei-Heidarin, H. R., Ziaee, A. A., Amoozegar, M. A., Cheburkin, Y. and Budisa, N.

Synthetic Biology of Proteins: Tuning GFP´s Folding and Stability with Fluoroproline

PLoSONE 2008, 3

Steiner, T., Hess, P., Bae, J. H., Moroder, L. and Budisa, N.

Efficient N-terminal Glycoconjugation of Proteins by the N-End Rule

ChemBioChem 2008, 9, 1220-1224

Merkel, L., Beckmann, H. S. G., Wittmann, V. and Budisa, N.

Photostability of Green and Yellow Fluorescent Proteins with fluorinated Chromophores, investigated by Fluorescence Correlation Spectroscopy

Biophys.Chem. 2008, 136, 38-43

Veetil, S. K., Budisa, N. and Jung, G.

Docking of Tryptophan analogues to Tryptophanyl-tRNA Synthetase; implication for non-natural amino acid incorporation.

Biol. Chem. 2008,389,1173-1182

Azim, M. K. and Budisa, N.

Advances in the stereoselective synthesis of Homopropargylglycine

J. Peptide Sci. 2008,14,49

Dong, S. L., Merkel, L., Musiol, H. J., Budisa, N. and Moroder, L.

Convenient Syntheses of Homopropargylglycine

J. Peptide Sci. 2008,14,1148-1150

Dong, S. L., Merkel, L., Moroder, L. and Budisa, N.

Intracellular uptake and inhibitory activity of aromatic fluorinated amino acids in human breast cancer cells

ChemMedChem 20083,1149-1456

Giese, C., Lepthien, S., Metzner, L., Brandsch, M., Budisa, N. and Lilie, H.

Azatryptophans endow proteins with intrinsic blue fluorescence

Proc. Natl. Acad. Sci. USA 2008105,16095-16100

Lepthien, S., Hoesl, M. G., Merkel, L. and Budisa, N.

Expanding the genetic code of Saccharomyces cerevisiae with methionine analogs

Yeast 200825,775-786

Wiltschi, B., Wenger, W., Nehring, S. and Budisa, N.

Basic Requirements for reprogramming intracellular protein translation

Molecular Interactions - Bringing Chemistry to Life. Proceedings of the International Beilstein Workshop 2007,93-109

Wiltschi, B. and Budisa, N.

Back into the Fold - Protein Engineering Protocols (Series: Methods in Molecular Biology, Vol. 352)

Appl. Microbiol. Biotechnol. 200673,740-754

Budisa, N.

Natural history and experimental evolution of the genetic code

Appl. Microbiol. Biotechnol. 200774,739

Wiltschi, B. and Budisa, N.

In Vivo Chemoenzymatic Control of N-terminal Processing in Recombinant Human Epidermal Growth Factor

ChemBioChem 20078,2227-2232

Merkel, L., Wiltschi, B., Cheburkin, Y. B. and Budisa, N.

High performance fluorescence scanning of tryptophan with Tecan´s Safire microplate reader

Tecan Journal 20062,13-16

Lepthien, S. and Budisa, N.

Veränderung des genetischen Codes

BIOspektrum 200612,41-43

Merkel, L. and Budisa, N.

In vivo engineering of proteins with nitrogen-containing tryptophan analogs

Appl. Microbiol. Biotechnol. 200673,740-754

Lepthien, S., Bolic, B., Wiltschi, B. and Budisa, N.

Aminotryptophan-containing barstar: Structure-functions trade-off in protein design and engineering with an expanded genetic code

Biochem. Biophys. Acta - Proteomics 1674 20061764,1147-1158

Rubini, M., Lephtien, S., Golbik, R. and Budisa, N.

Engineering the Genetic Code - Expanding the Amino Acid Repertoire for the Design of Novel Proteins

2005,WILEY-VHC

Budisa, N.

Evolutionary Methods in Biotechnology. Clever Tricks for Directed Evolution

Angewandte Chemie 2005, 117, 3175-3176

Budisa, N.

Cross-reactivity studies of rMOG(ED) with synthetic putative autoantigens CSF114(Glc) and [N-31(Glc)]hMOG(30-50) in multiple sclerosis patients´sera

Understanding Biology Using Peptides 2005, American Peptide Society, 769-770

Mulinacci, B., Breithaupt, C., Pal, P. P., Budisa, N., Pazzagli, M., Mazzanti, M., Rovero, P., Moroder, L. and Papini, A. M.

Structural and spectral response of the Aequorea victoria Green Fluorescent Proteins to the chromophore fluorination

Biochemistry 2005, 44, 3663-3672

Pal, P. P., Bae, J. H., Azim, M. K., Hess, P., Huber, R., Moroder, L. and Budisa, N.

Peptidyl Prolyl cis/trans-Isomerases: Comparative Reactivities of Cyclophilins, FK506-Binding Proteins, and Parvulins with Fluorinated Oligopeptide and Protein Substrates

Biochemistry 2005, 44, 16026-16034

Golbik, R., Chao Yu, C., Weyher-Stingl, E., Huber, R., Moroder, L., Budisa, N. and Schiene-Fischer, C.

Adding new tools to expressed protein ligation

ChemBioChem 2004, 5, 1176-1179

Budisa, N.

Designing novel spectral classes of proteins with tryptophan-expanded genetic code

Biol. Chem. 2004, 385, 893-904

Pal, P. P. and Budisa, N.

Prolegomena to future efforts on genetic code engineering by expanding its amino acid repertoire

Angew. Chem. Int. Ed. 2004, 43, 3387-3428

Budisa, N.

Probing the Role of Tryptophans in Aequorea victoria Green Fluorescent Proteins with an Expanded Genetic Code

Biol. Chem. 2004, 385, 191-202

Budisa, N., Pal, P. P., Alefelder, S., Birle, P., Krywcun, T., Rubini, M., Wenger, W., Bae, J. H. and Steiner, S.

Crystallographic Evidence for Isomeric Chromophores in 3-Fluorotyrosyl-Green Fluorescent Protein

ChemBioChem 2004, 5, 720-722

Bae, J. H., Pal, P. P., Moroder, L., Huber, R. and Budisa, N.

Efforts toward the design of "Teflon" proteins: In vivo translation with trifluorinated leucine and methionine analogues

Chem. & Biodiv. 2004, 1, 1465-1475

Budisa, N., Pipitone, O., Slivanowiz, I., Rubini, M., Pal, P. P., Holak, T. A. and Gelmi, M. L.

Expansion of the Genetic Code Enables Design of a Novel "Gold" Class of Green Fluorescent Proteins

J. Mol. Biol. 2003, 328, 977-1202

Bae, J., Rubini, M., Jung, G., Wiegand, G., Seifert, M. H. J., Azim, M. K., Kim, J. S., Zumbusch, A., Holak, T. A., Moroder, L., Huber, R. and Budisa, N.

Cell free Protein Expression

2003, Springer Verlag

Budisa, N.

Global replacement of tryptophan with amino-tryptophans generates non-invasive protein-based optical pH sensors

Angew. Chem. Int. Ed. Engl. 2002, 41, 4066-4069

Budisa, N., Rubini, M., Bae, J. H., Weyher, E., Wenger, W., Golbik, R., Huber, R. and Moroder, L.

Slow Conformational Exchange Processes in Green Fluorescent Protein Variants evidenced by NMR Spectroscopy

J. Am. Chem. Soc. 2002, 124, 7932-7942

Seifert, M. H. J., Ksiazek, D., Smialowski, P., Azim, M. K., Budisa, N. and Holak, T. A.

Proteins with beta-(thienopyrrolyl)alaninies as alternative chromophores and pharmaceutically active amino acids

Prot. Sci. 2001, 10, 1281-1292

Budisa, N., Alefelder, S., Bae, J. H., Golbik, R., Minks, C., Huber, R. and Moroder, L.

Incorporation of beta-selenolo[3,2-b]pyrrolyl-alanine into proteins for phase determination in protein X-ray crystallography

J. Mol. Biol. 2001, 309, 925-936

Bae, J. H., Alefelder, S., Kaiser, J. T., Friedrich, R., Moroder, L., Huber, R. and Budisa, N.

Fluoroprolines as tools for protein design and engineering

Angew. Chem. Int. Ed. Engl. 2001, 40, 923-925

Renner, C., Alefelder, S., Bae, J. H., Budisa, N., Huber, R. and Moroder, L.

Synthesis of b-(1-Azulenyl)-L-Alanine as a potential blue-coloures fluorescent tryptophan analogue and its use in peptide synthesis

J. Peptide Sci. 2000, 6, 139-144

Loid, G., Musiol, H.-J., Budisa, N., Huber, R., Poirot, S., Fourmy, D. and Moroder, L.

Towards new protein engineering: I vivo building and folding of protein shuttles for drug delivery and targeting by the selective pressure incorporation (SPI) method

Tetrahedron 2000, 56, 9431-9442

Minks, C., Alefelder, S., Moroder, L., Huber, R. and Budisa, N.

Non-invasive tracing of recombinant proteins with "fluorophenylalanine-fingers

Anal. Biochem. 2000, 284, 29-34

Minks, C., Huber, R., Moroder, L. and Budisa, N.

Toward the experimental codon reassignment in vivo: protein building with an expanded amino acid repertoire

FASEB 1999, 13, 41-51

Budisa, N., Minks, C., Alefelder, S., Wenger, W., Dong, F., Mororder, L. and Huber, R.

Atomic mutations at the single tryptophan residue of human recombinant annexin V: Effects on structure, stability and activity

Biochemistry 1999, 38, 10649-10659

Minks, C., Huber, R., Moroder, L. and Budisa, N.

Structure and evolution of the genetic code viewed from the perspective of the experimentally amino acid repertoire in vivo

Cell. Mol. Life Sci. 1999, 55, 1626-1635

Budisa, N., Mororder, L. and Huber, R.

Probing protein stability with non-natural amino acids

Croat. Chem. Acta 1998, 71, 179-187

Budisa, N. and Pifat, G.

Atomic mutations in annexin V. Thermodynamic studies of isomorphous protein variants

Eur. J. Biochem. 1998, 253, 1-9

Budisa, N., Huber, R., Minks, C., Golbik, R., Weyher, E. and Moroder, L.

Chalcogen-analogs of amino acids. Their use in X-ray crystallographic and folding studies of peptides and proteins

Biol. Chem. 1997, 378, 211-218

Besse, D., Budisa, N., Karnbock, W. M. C., Musiol, H. J., Pergoraro, S., Siedler, F., Weyher, E. and Moroder, L.

Bioincorporation of telluromehionine into proteins: A promising new approach for X-ray structure analysis of proteins

J. Mol. Biol. 1997, 270, 616-623

Budisa, N., Karnbock, W., Steinbacher, S., Humm, A., Prade, L., Neuefeind, T., Moroder, L. and Huber, R.

Phage P22 Tailspike protein: Crystal structure of the head binding domain at 2.3 Å, fully refined structure of the endorhamnosidase at 1.56 Å resolution, and the molecular basis of O-antigen recognition and cleavage

J. Mol. Biol. 1997, 267, 865-880

Steinbacher, S., Miller, S., Baxa, U., Budisa, N., Weintraub, A., Seckler, R. and Huber, R.

On the role of conserved methionine in metzincins: an active selenomethionine 215-variant of leukocyte collagenase with similar structure as the wild-type form

J. Prot. Chem. 1997, 16, 637-650

Pieper, M., Betz, M., Budisa, N., Gomis-Ruth, F.-X., Bode, W. and Tschesche, H.

A new efficient synthesis of acetyltelluro- and acetylselenomethionine and their use in the biosynthesis of heavy-atom protein analogues

J. Am. Chem. Soc. 1996, 118, 913-914

Karnbock, W., Weyher, E., Budisa, N., Huber, R. and Moroder, L.

High level biosynthetic substitution of methionine in proteins by its analogues 2-aminohexanoic acid, selenomethionine, telluromethionine and ethionine in Escherichia coli

Eur. J. Biochem. 1995, 230, 788-796

Budisa, N., Steipe, B., Demange, P., Eckerskorn, C., Kellermann, J. and Huber, R.

Enterotoxicity of Staphylococcus aureus strains isolated from foods and clinical materials

Food Technol. Biotechnol. 1992, 30, 79-82

Budisa, N., Benussi, A., Mihokovic, V. and Shinagawa, K.

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