Publications
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Schipp, C. J., Ma, Y., Al-Shameri, A. Contestabile, R, Budisa*, N. and DiSalvo*, M.L.
An Engineered Escherichia coli Strain with Synthetic Metabolism for In-cell Production of Translationally Active Methionine Derivatives
ChemBioChem 2020 , 21(24), 3525-3538
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Tseng, H.W., Baumann, T., Sun, H., Wang, Y. S., Ignatova, Z. and Budisa, N.
Expanding the Scope of Orthogonal Translation with Pyrrolysyl-tRNA Synthetases Dedicated to Aromatic Amino Acids.
Molecules 2020 , 25(19), 4418
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Schmitt, F.-J., Frielingsdorf, S. Friedrich, T. and Budisa, N.
Courses Based on iGEM/BIOMOD Competitions Are the Ideal Format for Research‐Based Learning of Xenobiology.
ChemBioChem 2020 , Online ahead of print
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Agostini, F., Sinn, L., Petras, D., Schipp, C.J., Kubyshkin, V., Berger, A.A., Rappsilber, J., Dorrestein, P.C., Budisa, N. and Koksch, B.
Multi-omics analysis provides insight into the laboratory evolution of Escherichia coli towards the metabolic usage of fluorinated indoles.
ACS Cent. Sci 2020 , Online ahead of print
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Deng, J., Viel, J-H., Kubyshkin, V., Budisa, N. and Kuipers, O.
Conjugation of Synthetic Polyproline Moietes to Lipid II Binding Fragments of Nisin Yields Active and Stable Antimicrobials.
Front. Microbiol. 2020 , 11:575334
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Heidari-Karbalaei, H. R. and Budisa, N.
Combating antimicrobial resistance with new-to-nature lanthipeptides created by genetic code expansion.
Front. Microbiol. 2020 , 1:590522
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Sun, H., Jia, H., Ramirez-Diaz, D. A., Budisa, N. and Schwille, P.
Fine‐tuning protein self‐organization by orthogonal chemo‐optogenetic tools
Angew Chem Int Ed Engl 2020 , Online ahead of print
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Perfumo, A., von Sass, G.J.F., Nordmann, E.L., Budisa, N. and Wagner, D.
Discovery and characterization of a new cold-active protease from an extremophilic bacterium via comparative genome analysis and in vitro expression
Front. Microbiol. 2020 , 11:881
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Budisa, N., Kubyshkin, V. and Schmidt, M.
Lauster, D., Klenk, S., Ludwig, K.; &, e. al, Budisa, N., Netz., R., Böttcher, C., Liese, S., Herrmann, A. and Hackenberger, C.P.
Phage capsid nanoparticles with defined ligand arrangement block influenza virus entry - an antiviral strategy
Nat Nanotechnol 2020 , 15, 373-379
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Schneider, T., Brüssow, N., Yuvanc, A. and Budisa, N.
Synthesis of New Aza- and Thia-Crown Ether Based Amino Acids
ChemistrySelect 2020 ,5(9), 2854-2857
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Schmitt, F.-J., Budisa, N. and Krause-Rehberg, R.
Student-centred research-based projects are highly motivating
84th DPG Spring Meeting of the German Physical Society 2020
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Budisa, N. and Schmidt, M.
Alternative Biofacts - Life as we don't (yet) know it
Art as we don’t know it. 2020 , Book published by Aalto University Publication Series: Helsinki; pp 75-83
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Kubyshkin, V., Budisa, N.
The Alanine World Model for the Development of the Amino Acid Repertoire in Protein Biosynthesis
Int. J. Mol. Sci. 2019 ,20(21), e5507
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Kubyshkin, V., Grage, S.L., Ulrich, A.S., Budisa N.
Bilayer thickness determines the alignment of model polyproline helices in lipid membranes
Phys Chem Chem Phys. 2019 ,21(40), 22396-22408
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Diwo. C, Budisa, N.
Alternative Biochemistries for Alien Life: Basic Concepts and Requirements for the Design of a Robust Biocontainment System in Genetic Isolation
Genes 2019 ,10(1),17
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Gerrits, M., Merk, H., Budisa, N.
Site-Specific Chemoselective Pyrrolysine Analogues Incorporation Using the Cell-Free Protein Synthesis System
ACS Synth. Biol. 2019 ,8(2), 381-390
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Nojoumi, S., Ying, M., Schwagerus, S., Hackenberger, C.P.,R., Budisa, N.
In-Cell Synthesis of Bioorthogonal Alkene Tag S-Allyl-Homocysteine and Its Coupling with Reprogrammed Translation
Int. J. Mol. Sci. 2019 ,20(2), 2299
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Baumann, T., Hauf, M., Schildhauer, F., Eberl, K.B., Durkin, P.M., Deniz, E., Löffler, J.G., Acevedo-Rocha, C.G., Jaric, J., Martins, B.M., Dobbek, H., Bredenbeck, J., Budisa, N.
Site-Resolved Observation of Vibrational Energy Transfer Using a Genetically Encoded Ultrafast Heater
Angew. Chem. Int. Ed. Engl. 2019 ,58(9), 2899-2903
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Baumann, T., Hauf, M.; Richter, F., Albers, S., Möglich, A., Ignatova, Z., Budisa, N.
Computational Aminoacyl-tRNA Synthetase Library Design for Photocaged Tyrosine
Int. J. Mol. Sci. 2019 ,20(9), 2343
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Kubyshkin, V., Budisa, N.
Promotion of the collagen triple helix in a hydrophobic environment
Org. Biomol. Chem. 2019 ,17(2), 2502-2507
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Kubyshkin, V., Budisa, N.
Anticipating alien cells with alternative genetic codes: away from the alanine world!
Curr. Opin. Biotechnol. 2019 ,60, 242-249
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Schneider, T., Budisa, N.
Expanding the DOPA Universe with Genetically Encoded, Mussel-Inspired Bioadhesives for Material Sciences and Medicine
ChemBioChem 2019 2019 ,20, 1-29
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Schneider, T.; Budisa, N.
Synthesis of a new metal chelating amino acid: Terpyridyl-alanine
Tetrahedron Lett. 2019 ,2018(19), 2053-2063
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Budisa, N., B Fehse, B.; Müller-Röber, B., Reich, J.; Walter, J.
Accounting for a High Technology - Fourth Genetic Technology Report of Interdisciplinary Research Group of the Berlin-Brandenburg Academy of Sciences and Humanities
Nomos Verlag, Germany 2018 ,203-236
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Ma, Y., Salvo, M. L., Budisa, N.
Self-Directed in Cell Production of Methionine Analogue Azidohomoalanine by Synthetic Metabolism and Its Incorporation into Model Proteins
Mol. Biol. 2018 (1),127-135
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Stempel E., Kaml R. F., Budisa, N., Kalesse, M.
Painting argyrins blue: Negishi cross-coupling for synthesis of deep-blue tryptophan analogue ß-(1-azulenyl)-l alanine and its incorporation into argyrin C.
Bioorg. Med. Chem. 2018 , in Press.
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Biava, H.; Schreiber, T., Katz, S., Vo¨ller, J-S., Stolarski, M., Schulz, C., Michael, N., Budisa, N., Kozuch, J., Tillmann, U., Hildebrandt, P.
Long-Range Modulations of Electric Fields in Proteins
J. Phys. Chem. B 2018 B122(35),8330-8342
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Schneider, T., Kubyshkin, V., Budisa, N.
Synthesis of a Photo-Caged DOPA Derivative by Selective Alkylation of 3,4-Dihydroxybenzaldehyde
Eur. J. Org. Chem. 2018 2018(18),2053-2063
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Nickling, J. H., Baumann, T., Schmitt, F. J., Bartholomae, M., Kuipers, O. P., Friedrich, T., Budisa, N.
Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids.
J. Vis. Exp. 2018 e57551(135)
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Baumann, T., Schmitt, F. J., Pelzer, A., Spiering, V. J., Freiherr von Sass, G. J., Friedrich, T., Budisa, N.
Engineering 'Golden' Fluorescence by Selective Pressure Incorporation of Non-canonical Amino Acids and Protein Analysis by Mass Spectrometry and Fluorescence.
J. Vis. Exp. 2018 e57017(134)
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Kubyshkin, V., Grage, S. L., Bürck, J., Ulrich, A. S., Budisa, N.
Bartholomae, M., Baumann, T., Nickling, J.H., Peterhoff, D., Wagner, R., Budisa, N., Kuipers, O. P.
Expanding the genetic code of Lactococcus lactis and Escherichia coli to incorporate non-canonical amino acids for production of modified lantibiotics
Front. Microbiol. 2018 9,657
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Kubyshkin, V., Budisa, N.
Exploring hydrophobicity limits of polyproline helix with oligomeric octahydroindole-2-carboxylic acid
J. Pept. Sci. 2018 24(4-5),e3076
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Kubyshkin, V., Pridma, S., Budisa, N.
“Comparative effects of trifluoromethyl-and methyl-group substitutions in proline
New J. Chem. 2018 , 42, 13461-13470
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Serikawa, T., Spanos, C., vonHacht, A., Budisa, N., Rappsilber, J., Kurreck, J.“Comparative effects of trifluoromethyl-and methyl-group substitutions in proline
Comprehensive identification of proteins binding to RNA G-quadruplex motifs in the 5' UTR of tumor-associated mRNAs
Biochimie 2018 , 144, 169-184
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Jan-Stefan Völler, Morana Dulic, Ulla I. M. Gerling-Driessen, Hernan Biava, Tobias Baumann, Nediljko Budisa*, Ita Gruic-Sovulj*, and Beate Koksch*
Discovery and Investigation of Natural Editing Function against Artificial Amino Acids in Protein Translation
ACS Cent. Sci. 2017 ,3 (1),73-80
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Federica Agostini, Jan-Stefan Völler, Beate Koksch, Carlos G. Acevedo-Rocha, Vladimir Kubyshkin and Nediljko Budisa
Biocatalysis with Unnatural Amino Acids: Enzymology Meets Xenobiology
Angew. Chem. Int. Ed. 2017, 56, 2-26
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Vladimir Kubyshkin and Nediljko Budisa
Allison Ann Berger, Jan-Stefan Völler, Nediljko Budisa and Beate Koksch
Deciphering the Fluorine Code—The Many Hats Fluorine Wears in a Protein Environmenty
Acc. Chem. Res. 2017, 50(9), 2093-2103
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Vladimir Kubyshkin and Nediljko Budisa
Synthetic alienation of microbial organisms by using genetic code engineering: Why and how?
Biotechnol. J. 2017, 12(8),
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Vladimir Kubyshkin and Nediljko Budisa
Construction of a polyproline structure with hydrophobic exterior using octahydroindole-2-carboxylic acid
Org. Biomol. Chem. 2017, 15, 619-627
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Jan-Stefan Völler, M. G. Hoesl and Prof. Dr. Nediljko Budisa
Künstliche Evolution des genetischen Codes von Mikroorganismen (In German)
BIOspektrum 2017, 21, 146-149
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Jan-Stefan Völler, Hernan Biava, Peter Hildebrandt and Nediljko Budisa
An expanded genetic code for probing the role of electrostatics in enzyme catalysis by vibrational Stark spectroscopy
Biochim. Biophys. Acta 2017, 1861, 3053-3059
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Jan-Stefan Völler and Nediljko Budisa
Coupling genetic code expansion and metabolic engineering for synthetic cells
Curr. Op. Biotechnol. 2017, 48, 1-7
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Markus Schmidt, Lei Pei and Nediljko Budisa
Xenobiology: State-of-the-Art, Ethics, and Philosophy of New-to-Nature Organisms
Synthetic Biology-Metabolic Engineering 301-315
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Mykhailiuk, P., Kishko, I., Kubyshkin, V., Budisa, N. and Cossy, J.
Selective 19F-Labeling of Functionalized Carboxylic Acids with Difluoromethyl Diazomethane (CF2HCHN2)
Chem. Eur. J. 2017, 23, 13279-13283
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Kubyshkin, V., Acevedo-Rocha, C. G. and Budisa, N.
Baumann, T., Nickling, J. H., Bartholomae, M., Buivydas, A., Kuipers, O. P. and Budisa, N.
Prospects of In vivo Incorporation of Non-canonical Amino Acids for the Chemical Diversification of Antimicrobial Peptides
Front. Microbiol. 2017, 8, 124
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Schumacher, D., Lemke, O., Helma, J., Gerszonowicz, L., Waller, V., Stoschek, T., Durkin, P. M., Budisa, N., Leonhardt, H., Keller, B. and Hackenberger, C.
Broad substrate tolerance of tubulin tyrosine ligase enables one-step site-specific enzymatic protein labeling
Chem. Sci. 2017, 8, 3471-3478
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Schneider, T., Martin, J., Durkin, P. M., Kubyshkin, V. and Budisa, N.
The Regioselective Synthesis of o-Nitrobenzyl DOPA Derivatives
Synthesis 2017, 49, 2691-2699
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Mykhailiuk, P., Kubyshkin, V., Bach, T. and Budisa, N.
Peptidyl-Prolyl Model Study: How Does the Electronic Effect Influence the Amide Bond Conformation?
J. Org. Chem. 2017, 82(17), 8831-8841
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Kubyshkin, V. and Budisa, N.
Hydrolysis, polarity, and conformational impact of C-terminal partially fluorinated ethyl esters in peptide models
Beilstein J. Org. Chem. 2017, 2442-2457
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Serikawa T., Spanos C., von Hacht A., Budisa N., Rappsilber J. Kurreck J.
Comprehensive identification of proteins binding to RNA G-quadruplex motifs in the 5' UTR of tumor-associated mRNAs
Biochemie 2017, 144, 2442-2457
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Völler, J.-S., To, T., Biava, H., Koksch, B. and Budisa, N.
Global substitution of hemeproteins with noncanonical amino acids in Escherichia coli with intact cofactor maturation machinery
Enzyme Microb. Technol. 2017, 106, 55-59
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Hauf, M., Richter, F., Schneider, T., Faidt, T., Martins, B. M., Baumann, T., Durkin, P., Dobbek, H., Jacobs, K., Moeglich, A. and Budisa, N.
Photoactivatable Mussel-Based Underwater Adhesive Proteins by an Expanded Genetic Code
ChembioChem 2017, 18, 1819-1823
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Exner, M. P., Kuenzl, T., Schwagerus, S., To, T., Ouyang, Z., Hoesl, M. G., Lensen, M. C., Hackenberger, C. P. R., Panke, S. and Budisa, N.
Design of S-Allylcysteine in Situ Production and Incorporation Based on a Novel Pyrrolysyl-tRNA Synthetase Variant
ChembioChem 2017, 85-90
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Worst, E. G., Exner, M. P., De Simone, A., Schenkelberger, M., Noireaux, V., Budisa, N. and et al.
Residue-specific Incorporation of Noncanonical Amino Acids into Model Proteins Using an Escherichia coli Cell-free Transcription-translation System
J. Vis. Exp. 2016, e54273
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Köhling, S., Exner, M. P., Nojoumi, S., Schiller, J., Budisa, N. and Rademann, J.
One-Pot Synthesis of Unprotected Anomeric Glycosyl Thiols in Water for Glycan Ligation Reactions with Highly Functionalized Sugars
Angew. Chem. Int. Ed. 2016, 55 15510-15514
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De Simone, A., Hoesl, M. G. and Budisa, N.
Congeneric lipases with improved catalytic activity and substrate accessibility / Engineering lipases with an expanded genetic code
Applied Biocatalysis From Fundamental Science to Industrial Applications 2016, Wiley-VCH
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Avcilar-Kucukgoze, I., Bartholomäus, A., Cordero Varela, J., Kaml, R., Neubauer, P., Budisa, N. and Ignatova, Z.
Discharging tRNAs: a tug of war between translation and detoxification in Escherichia coli
Nucleic Acids Res. 2016, 44, 8324-8334
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Acevedo-Rocha, C. and Budisa, N.
Xenomicrobiology: a roadmap for genetic code engineering
Microb. Biotechnol. 2016, 9, 666-676
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Exner, M. P., Köhling, S., Rivollier, J., Gosling, S., Srivastava, P., Palyancheva, Z. I., Herdewijn, P., Heck, M.-P., Rademann, J. and Budisa, N.
Incorporation of Amino Acids with Long-Chain Terminal Olefins into Proteins
Molecules 2016, 21, 287
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Kubyshkin, V. and Budisa, N.
cis–trans-Amide isomerism of the 3,4-dehydroproline residue, the ‘unpuckered’ proline
Beilstein J. Org. Chem. 2016, 12, 589-593
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Kubyshkin, V., Durkin, P. M. and Budisa, N.
Energetic contribution to both acidity and conformational stability in peptide models
New J. Chem. 2016, 40, 5209-5220
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Engelhard, M., Boelker, M., Budisa, N. and Winter, G.
Boelker, M., Engelhard, M. and Budisa, N.
Husak, A., Noichl, B., Sumanovac-Ramljak, T., Sohora, M., Skalamera, D., Budisa, N. and Basaric, N.
Photochemical formation of quinone methides from peptides containing modified tyrosine
Organic & Biomolecular Chemistry 2016, 14, 10894-10905
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Durkin, P. M. and Budisa, N.
Design and Application of Autofluorescent Proteins by Biological Incorporation of Intrinsically Fluorescent Noncanonical Amino Acids
Fluorescent Analogs of Biomolecular Building Blocks 2016, Wiley, 91-124
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Baumann, T., Exner, M. P. and Budisa, N.
Orthogonal Protein Translation Using Pyrrolysyl-tRNA Synthetases for Single - and Multiple-Noncanonical Amino Acid Mutagenesis
Adv. Biochem. Eng. Biotechnol. 2016, Springer, 1-19
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Baumann, T., Exner, M. P. and Budisa, N.
Towards reassignment of the AUG Methionine codon to two different non-canonical amino acids in bacterial translation
Croatica Chemica Acta 2016, 89, 1-19
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Kuthning, A., Durkin, P., Oehm, S., Hoesl, M., Budisa, N. and Süssmuth, R.
Towards Biocontained Cell Factories: An Evolutionarily Adapted Escherichia coli Strain Produces a New-to-nature Bioactive Lantibiotic Containing Thienopyrrole-Alanine
Scientific Reports 2016, 6
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Nischan, N., Herce, H., Natale, F., Bohlke, N., Budisa, N., Cardoso, M. C. and Hackenberger, C. P. R.
Covalent Attachment of Cyclic TAT Peptides to GFP Results in Protein Delivery into Live Cells with Immediate Bioavailability
Angew. Chem. Int. Ed. Eng. 2015, 54, 1950-1953
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Dietz, D., Kubyshkin, V. and Budisa, N.
Applying ?-substituted prolines in the foldon peptide: polarity contradicts preorganization
ChembioChem 2015, 16, 403-406
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Kubyshkin, V., Afonin, S., Kara, S., Budisa, N., Mykhailiuk, P. K. and Ulrich, A. S.
?-(S)-Trifluoromethyl proline: Evaluation as a structural substitute of proline for solid state 19F-NMR peptide studies
Org. Biomol. Chem. 2015, 13, 3171-3181
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Budisa, N. and Hümpel, A.
Synthetic Biology: Is a new kind of Biological Engineering emerging?
Third gene technology report: Analysis of a high-tech sector. 2015
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Völler, J., Biava, H., Koksch, B., Hilderbrandt, P. and Budisa, N.
Orthogonal Translation Meets Electron Transfer: In Vivo Labeling of Cytochrome c for Probing Local Electric Fields
ChembioChem 2015, 16, 742-745
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Budisa, N.
Xenobiologie, künstliches Leben und genetische Firewall
Grenzüberschreitungen - Synthetische Biologie im Dialog. 2015, Verlag Karl Alber
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Jaric, J. and Budisa, N.
Design of orthogonal pairs for protein translation: selection systems for genetically encoding noncanonical amino acids in E. coli
Hydrocarbon and Lipid Microbiology Protocols: Cultivation 2015, Springer-Verlag
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Mühlberg, M., Hoesl, M. G., Kuehne, C., Dernedde, J., Budisa, N. and Hackenberger, C. P. R.
Orthogonal dual-modification of proteins for the engineering of multivalent protein scaffolds
Beilstein J. Org. Chem. 2015, 11, 784-791
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Müller-Werkmeister, H. M., Essig, M., Durkin, P., Budisa, N. and Bredenbeck, J.
Towards Direct Measurement of Ultrafast Vibrational Energy Flow in Proteins
Ultrafast Phenomena XIX 2015
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Albrecht, M., Lippach, A., Exner, M. P., Jerbi, J., Springborg, M., Budisa, N. and Wenz, G.
Site-specific conjugation of 8-ethynyl-BODIPY to a protein by [2+3] cycloaddition
Org. Biomol. Chem. 2015, 13, 6728-6736
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Worst, E. G., Exner, M. P., De Simone, A., Schenkelberger, M., Noireaux, V., Budisa, N. and Ott, A.
Cell-free expression with the toxic amino acid canavanine
Bioord. Med. Chem. Lett. 2015, 25, 3658-3660
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Noichl, B. P., Durkin, P. M. and Budisa, N.
Towards intrinsically colored peptides: The synthesis and investigation of the spectral properties of methylated azatryptophans in Trp-cage mutants
Biopolymers Peptide Science 2015, 104, 585-600
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Hoesl, M. G., Oehm, S., Durkin, P., Darmon, E., Peil, L., Aerni, H.-R., Rappsilber, J., Rinehart, J., Leach, D., Söll, D. and Budisa, N.
Chemical evolution of a bacterial proteome
Angew. Chem. Int. Ed. Engl. 2015, 54, 10030-10034
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Eisenreich, W., Joshi, M., Illarionov, B., Kacprzak, S., Lukaschek, M., Kothe, G., Budisa, N., Fischer, M., Bacher, A. and Weber, S.
Strategy for Enhancement of 13C-Photo-CIDNP NMR Spectra by Exploiting Fractional 13C-Labeling of Tryptophan
J. Phys. Chem. 2015, 119(43), 13934-13943
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Ma, Y., Thota, B. N. S., Haag, R. and Budisa, N.
Dendronylation: Residue-specific chemoselective attachment of oligoglycerol dendrimers on proteins with noncanonical amino acids
Bioorg. Med. Chem. Lett. 2015, 25, 5247-5249
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Doerfel, L. K., Wohlgemuth, I., Kubyshkin, V., Starosta, A. L., Wilson, D. N., Budisa, N. and V., R. M.
Entropic Contribution of Elongation Factor P to Proline Positioning at the Catalytic Center of the Ribosome
J. Am. Chem. Soc. 2015, 137, 12997-13006
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Budisa, N.
Life at the Speed of Light– From the From the Double Helix to the Dawn of Digital Life. By J. Craig Venter
Angew. Chem. Int. Ed. Engl. 2014, 53, 9421-9422
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Pournejati, R., Karbalaei-Heidari, H. R. and Budisa, N.
Secretion of Recombinant Archeal lipase Mediated by SVP2 Signal Peptide in Escherichia coli and its optimization by Response Surface Methodology
Protein Expr. Purif. 2014, 101, 84-90
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Biava, H.,Budisa, N.
Evolution of fluorinated enzymes: An emerging trend for biocatalyst stabilization
Eng. Life. Sci. 2014, 14, 340-351
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Bohlke, N. and Budisa, N.
Sense codon emancipation for proteome-wide incorporation of noncanonical amino acids: rare isoleucine codon AUA as a target for genetic code expansion
FEMS Microbiol Lett. 2014, 351, 133-144
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Budisa, N.
Xenobiology, New-to-Nature Synthetic Cells and Genetic Firewall
Curr. Org. Chem. 2014, 18, 936-943
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Ma, Y., Biava, H., Contestabile, R., Budisa, N. and di Salvo, M. L.
Coupling Bioorthogonal Chemistries with Artificial Metabolism: Intracellular Biosynthesis of Azidohomoalanine and its Incorporation into Recombinant Proteins
Molecules 2014, 19, 1004-1022
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Heinen, A., Wanke, F., Moos, S., Attig, S., Luche, H., Pal, P., Budisa, N., Fehling, H., Waisman, A. and Kurschus, F.
Improved method to retain cytosolic reporter protein fluorescence while staining for nuclear proteins
Cytometry A. 2014, 85, 621-627
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Budisa, N. and Schulze-Makuch, D.
Supercritical Carbon Dioxide and Its Potential as a Life-Sustaining Solvent in a Planetary Environment
Life 2014, 4, 331-340
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Budisa, N., Kubyshkin, V. and Schulze-Makuch, D.
Supercritical Carbon Dioxide and Its Potential as a Life-Sustaining Solvent in a Planetary EnvironmentFluorine-Rich Planetary Environments as Possible Habitats for Life
Life 2014, 4, 374-385
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Al Toma, R. S., Kuthning, A., Exner, M. P., Denisiuk, A., Ziegler, J., Budisa, N. and Süssmuth, R. D.
Site-Directed and Global Incorporation of Orthogonal and Isostructural Noncanonical Amino Acids into the Ribosomal Lasso Peptide Capistruin
ChembioChem 2014, 16, 503-509
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Budisa, N.
Expanded genetic code for the engineering of ribosomally synthetized and post-translationally modified peptide natural products (RiPPs)
Biotechnol. 2013, 4, 591-598
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Seemann, K., Bauer, A., Kindervater, J., Meyer, M., Besson, C., Luysberg, M., Durkin, P., Pyckhout-Hintzen, W., Budisa, N., Georgii, R., Schneider, C. M. and Kogerler, P.
Polyoxometalate-stabilized, water dispersible Fe2Pt magnetic nanoparticles
Nanoscale 2013, 6, 2511-2519
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Staudt, H., Hoesl, M. G., Dreuw, A., Serdjukow, S., Oesterhelt, D., Budisa, N., Wachtveitl, J. and Grininger, M.
Directed Manipulation of a Flavoprotein Photocycle
Angew. Chem. Int. Ed. Engl. 2013, 52, 8463-8466
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Acevedo-Rocha, C., Hoesl, M., Nehring, S., Royter, M., Wolschner, C., Wiltschi, B., Antranikian, G. and Budisa, N.
Non-canonical amino acids as a useful synthetic biological tool for lipase-catalysed reactions in hostile environments
Catal. Sci. Technol. 2013, 3, 1198-1201
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Biava, H. and Budisa, N.
Biocatalytic synthesis of (2S)-5,5,5-trifluoroleucine and improved resolution into (2S,4S) and (2S,4R) diastereoisomers
Tetrahedron Letters 2013, 54, 3662-3665
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Biava, H. and Budisa, N.
Obtention of enantiomerically pure 5,5,5-trifluoro-L-isoleucine and 5,5,5-trifluoro-L-alloisoleucine
J. Fluorine Chem. 2013, 156, 372-377
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Budisa, N.
Oldach, F., Al-Toma, R., Kuthning, A., Caetano, T., Mendo, S., Budisa, N. and Süssmuth, R. D.
Congeneric Lantibiotics from Ribosomal In Vivo Peptide Synthesis with Noncanonical Amino Acids
Angew. Chem. 2012, 51, 415-418
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Artner, L. A., Merkel, L., Bohlke, N., Beceren-Braun, F., Weise, C., Dernedde, J., Budisa, N. and Hackenberger, C. P. R.
Site-selective modification of proteins for the synthesis of structurally defined multivalent scaffolds
Chem. Commun. 2012, 48, 522-524
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Hoesl, M. G. and Budisa, N.
Recent advances in genetic code engineering in Escherichia coli
Curr. Opin. Biotechnol. 2012, 23, 751-757
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Nehring, S., Budisa, N. and Wiltschi, B.
Performance analysis of orthogonal pairs designed for an expanded eukaryotic genetic code
PloSONE 2012, e31992
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Org. Biomol. Chem. 2012, 1, 7241-7261
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Design of protein congeners containing beta-cyclopropylalanine
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Org. Biomol. Chem. 2012, 10, 6629-6632
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Synthetische Biologie. Entwicklung einer neuen Ingenieurbiologie?
Berlin-Brandenburgische Akademie der Wissenschaften 2012
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Conjugation of Proteins by Installing BIO-Orthogonally Reactive Groups at Their N-Termini
PLoSONE 2012, e46741
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PLP-dependent Enzymes: a Powerful Tool for Metabolic Synthesis of. Non-canonical Amino Acids
Beilsten Symposium on Molecular Engineering and Control 2012, Logos Verlag Berlin
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Synthetic Biology of Autofluorescent Proteins
Fluorescent Proteins I - From Understanding to Design 2011, Springer-Verlag
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Code Engineering: Techniken und Anwendungen
Synthetische Biologie – acatech DISKUSSION XII 2011, Springer-Verlag
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Edwardraja, S., Sriram, S., Govindan, R., Budisa, N. and Lee, S. G.
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Expanding and Engineering the Genetic Code in a Single Expression Experiment
ChemBioChem 2011, 12, 552-555
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In Vivo Incorporation of Multiple Noncanonical Amino Acids into Proteins
Angew. Chem. Int. Ed. Engl. 2011, 50, 2896-2902
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Bioconjugation of L-3,4-Dihydroxyphenylalanine Containing Protein with a Polysaccharide
Bioconjugate Chehm. 2011, 22, 551-555
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Kernaussagen und Handlungsempfehlungen
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Blue fluorescent amino acids as in vivo building blocks for proteins
ChemBioChem 2010,11,305-314
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Mol. Biosyst. 2010,6,1630-1639
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Parallel Incorporation of Different Fluorinated Amino Acids: On the Way to "Teflon" Proteins
ChemBioChem 2010,11,1505-1507
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In Vivo Double and Triple Labeling of Proteins Using Synthetic Amino Acids
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Azatryptophans as tools to study polarity requirements for folding of green fluorescent protein
J. Peptide Sci. 2010,16,589-595
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ChemBioChem 2010, 11,2521-2524
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Fine tuning the N-terminal residue excision by methionine analogs
ChemBioChem 2009,10,217-220
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Cytometry Part A 2009,75,626-633
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Reviews in Fluoresence 2008, Springer New York,359-386
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Protein Engineering 2008,22,20-64
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Gene 2008,408,196-203
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Photostability of Green and Yellow Fluorescent Proteins with fluorinated Chromophores, investigated by Fluorescence Correlation Spectroscopy
Biophys.Chem. 2008, 136, 38-43
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Biol. Chem. 2008,389,1173-1182
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J. Peptide Sci. 2008,14,49
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ChemMedChem 20083,1149-1456
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Yeast 200825,775-786
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2005,WILEY-VHC
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ChemBioChem 2004, 5, 720-722
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J. Mol. Biol. 2003, 328, 977-1202
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J. Mol. Biol. 2001, 309, 925-936
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Fluoroprolines as tools for protein design and engineering
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Anal. Biochem. 2000, 284, 29-34
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FASEB 1999, 13, 41-51
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Atomic mutations at the single tryptophan residue of human recombinant annexin V: Effects on structure, stability and activity
Biochemistry 1999, 38, 10649-10659
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Structure and evolution of the genetic code viewed from the perspective of the experimentally amino acid repertoire in vivo
Cell. Mol. Life Sci. 1999, 55, 1626-1635
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Probing protein stability with non-natural amino acids
Croat. Chem. Acta 1998, 71, 179-187
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Atomic mutations in annexin V. Thermodynamic studies of isomorphous protein variants
Eur. J. Biochem. 1998, 253, 1-9
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Chalcogen-analogs of amino acids. Their use in X-ray crystallographic and folding studies of peptides and proteins
Biol. Chem. 1997, 378, 211-218
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Bioincorporation of telluromehionine into proteins: A promising new approach for X-ray structure analysis of proteins
J. Mol. Biol. 1997, 270, 616-623
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Phage P22 Tailspike protein: Crystal structure of the head binding domain at 2.3 Å, fully refined structure of the endorhamnosidase at 1.56 Å resolution, and the molecular basis of O-antigen recognition and cleavage
J. Mol. Biol. 1997, 267, 865-880
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On the role of conserved methionine in metzincins: an active selenomethionine 215-variant of leukocyte collagenase with similar structure as the wild-type form
J. Prot. Chem. 1997, 16, 637-650
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A new efficient synthesis of acetyltelluro- and acetylselenomethionine and their use in the biosynthesis of heavy-atom protein analogues
J. Am. Chem. Soc. 1996, 118, 913-914
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High level biosynthetic substitution of methionine in proteins by its analogues 2-aminohexanoic acid, selenomethionine, telluromethionine and ethionine in Escherichia coli
Eur. J. Biochem. 1995, 230, 788-796
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Enterotoxicity of Staphylococcus aureus strains isolated from foods and clinical materials
Food Technol. Biotechnol. 1992, 30, 79-82
